tbjournalТуберкулез и социально значимые заболеванияTuberculosis and socially significant diseases2413-03462413-0354ООО «Ин-Трендtbjournal-208Research ArticleВ ПОМОЩЬ ФТИЗИАТРУМолекулярные механизмы развития широкой лекарственной устойчивости и методы ее определения у M.tuberculosis.MOLECULAR MECHANISMS UNDERLYING EXTENSIVELY DRUG-RESISTANT TUBERCULOSIS, AND ASSAYS AVAILABLE FOR ITS DETECTIONНосоваЕ. Ю.NosovaE. Yu.

Носова Елена Юрьевна – ведущий научный сотрудник отдела проблем лабораторной диагностики туберкулёза и патоморфологии ГКУЗ «Московский городской научно-практический центр борьбы с туберкулёзом Департамента здравоохранения города Москвы», кандидат медицинских наук

107014, г. Москва, ул. Стромынка, д. 10Тел.: +7 (496) 603 30 33

ГикалоМ. Б.GikaloM. B.

Гикало Марина Борисовна – научный сотрудник отдела проблем лабораторной диагностики туберкулёза и патоморфологии ГКУЗ «Московский городской научно-практический центр борьбы с туберкулёзом Департамента здравоохранения города Москвы»

107014, г. Москва, ул. Стромынка, д. 10Тел.: +7 (496) 603 30 33

m-lle-marina@rambler.ruХахалинаА. А.HahalinaA. A.

Хахалина Анастасия Александровна – научный сотрудник отдела проблем лабораторной диагностики туберкулёза и патоморфологии ГКУЗ «Московский городской научно-практический центр борьбы с туберкулёзом Департамента здравоохранения города Москвы»

107014, г. Москва, ул. Стромынка, д. 10Тел.: +7 (496) 603 30 33

ГалкинаК. Ю.GalkinaK. Yu.

Галкина Ксения Юрьевна – ведущий научный сотрудник отдела проблем лабораторной диагностики туберкулёза и патоморфологии ГКУЗ «Московский городской научно-практический центр борьбы с туберкулёзом Департамента здравоохранения города Москвы», кандидат биологических наук

107014, г. Москва, ул. Стромынка, д. 10Тел.: +7 (496) 603 30 33

СафоноваС. Г.SafonovaS. G.

Сафонова Светлана Григорьевна – заведующая отделом проблем лабораторной диагностики туберкулёза и патоморфологии ГКУЗ «Московский городской научно-практический центр борьбы с туберкулёзом Департамента здравоохранения города Москвы», доктор биологических наук

107014, г. Москва, ул. Стромынка, д. 10Тел.: +7 (496) 603 30 33

safonova.s.g@inbox.ruГКУЗ «Московский городской научно-практический центр борьбы с туберкулёзом Департамента здравоохранения города Москвы»Russian Federation201322012025025660Copyright © Носова Е.Ю., Гикало М.Б., Хахалина А.А., Галкина К.Ю., Сафонова С.Г., 20252025Носова Е.Ю., Гикало М.Б., Хахалина А.А., Галкина К.Ю., Сафонова С.Г.Nosova E.Y., Gikalo M.B., Hahalina A.A., Galkina K.Y., Safonova S.G.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.tb-journal.ru/jour/article/view/208

В условиях повсеместного распространения лекарственно-устойчивых штаммов M. tuberculosis необходимо четкое понимание молекулярных основ развития устойчивости возбудителя к различным группам противотуберкулёзных препаратов. Устойчивость M. tuberculosis к фторхинолонам, как правило, вызвана различными мутациями в генах gyrA и gyrB, преимущественно в QRDR области. Устойчивость M. tuberculosis к аминогликозидам/капреомицину может быть связана с мутациями в гене rrs, промоторной области гена eis, а в редких случаях в гене tlyA. В клинической практике для выявления мутаций, связанных с устойчивостью M. tuberculosis к фторхинолонам и аминогликозидам/капреомицину могут быть использованы тест-системы ТБ-БИОЧИП2 (только для фторхинолонов) и GenoType MTBDRsl.

Considering the growing outspread of drug-resistant strains of M. tuberculosis, a clear understanding of the molecular mechanisms underlying drug-resistance of the pathogen to different groups of antibiotics is crucial. Fluoroquinolone-resistant M. tuberculosis generally harbor various mutations in the gyrA and gyrB genes, mostly in the QRDR region. M. tuberculosis resistance to aminoglycosides/ capreomycin is commonly associated with mutations in the rrs, gene, the eis promoter region, and occasionally in the tlyA gene. Two assays are available for the detection of mutations leading to fluoroquinolone and aminoglycoside resistance in clinical practice: the TB-BIOCHIP2 (only for fluoroquinolones) and the GenoType MTBDRsl.

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The authors declare that there are no conflicts of interest present.