A modified method of immunological diagnosis of intolerance to anti-tuberculosis drugs using additional stimulation of cultured lymphocytes with recombinant interleukin-2
https://doi.org/10.54921/2413-0346-2024-12-1-25-29
Abstract
This study is aimed to enhance the immune response to anti-TB drugs (anti-TBD) in vitro of circulating blood lymphocytes from patients with adverse reactions.
Methods. The immune response of lymphocytes to first-line anti-TBD was studied in 7 patients with hepatotoxic reactions (test group) and 13 patients without reactions (control group). The modified blast transformation test was used in a culture of lymphocytes in vitro; proliferative activity was increased using recombinant IL-2.
Results. The addition of IL-2 to control cultures with no anti-TBD had almost no effect on the blastogenic response of lymphocytes while the response to anti-TBD was increased 1,5–2 times. However, the stimulation index never exceeded 2. The addition of IL-2 to lymphocyte cultures of patients with hepatotoxic reactions caused an increase in the content of blasts to 4.5–5%. Different patients showed immune response simultaneously to 1 or more anti-TBD. In 3 cases the lymphocytes reacted to 3 drug combinations: R+Z+E, H+R+E and R+Z+H.
Conclusion. The modified blast transformation test is useful to identify the immune response of lymphocytes to anti-TBD in patients with hepatotoxic reactions.
About the Authors
M. M. Averbakh
Federal State Budgetary Scientific Institution «Central Tuberculosis Research Institute»
Russian Federation
Russian Federation
Moscow
G. A. Kosmiadi
Federal State Budgetary Scientific Institution «Central Tuberculosis Research Institute»
Russian Federation
Russian Federation
Moscow
V. Ya. Gergert
Federal State Budgetary Scientific Institution «Central Tuberculosis Research Institute»
Russian Federation
Russian Federation
Moscow
L. V. Panova
Federal State Budgetary Scientific Institution «Central Tuberculosis Research Institute»
Russian Federation
Russian Federation
Moscow
References
1. Абакушина Е.В., Маризина Ю.В., Неприна Г.С. Эффективность совместного применения IL-2 и IL-15 для активации цитотоксических лимфоцитов in vitro // Гены & клетки. – 2015. – Т. 10. – № 2. – С. 78-85.
2. Дубровская Н.А. Клинико-иммунологические проявления побочного действия рифампицина при лечении больных туберкулезом: Дисс. … канд. мед. наук. – М., 1984.
3. Курилин В.В., Хантакова Ю.Н., Облеухова И.А. Шевченко Ю.А., Куликова Е.В., Якушенко В.К., Соколов А.В., Сенников Ю.А. Стимуляция дендритными клетками in vitro противоопухолевой цитотоксической активности мононуклеарных клеток больных колоректальным раком // Мед. иммунология. – 2013. – Т. 15. – № 3. – С. 235-246.
4. Маслаускене Т.П., Николаева С.В. Побочное действие противотуберкулезных препаратов // Сибирский мед. журн. – 2005. – Т. 25. – С. 13-19.
5. Новиков П.Д., Новиков Д.К. Клиническая иммунопатология. – М., 2009. – 464 с.
6. Cheng M., Chen Y., Xiao W. et al. NK cell-based immunotherapy for malignant diseases // Cell. Mol. Immunol. – 2013. – Vol. 3. – № 10. – P. 1-23.
7. Lochmatter P., Zawodniak A., Pichler W.J. In vitro tests in drug hypersensitivity diagnosis // Immunol. Allergy Clin. N. Am. – 2009. – Vol. 29. – P. 537-554. doi:10.1016/j.iac.2009.04.009
8. Pichler W.J., Tilch J. The lymphocyte transformation test in the diagnosis of drug hypersensitivity // Allergy. – 2004. – Vol. 59. - P. 809-820.
9. Rosenberg S.A., Packard B.S., Aebersold P.M., Solomon D., Topalian S.L., Toy S.T., Simon P., Lotze M.T., Yang J.C., Seipp C..A. et al. Use of tumor-infiltrating lymphocytes and interleukin-2 in the immunotherapy of patients with metastatic melanoma. A preliminary report // N. Engl. J. Med. – 1988. – Vol. 319. – P. 1676-1680. doi: 10.1056/NEJM198812223192527
10. Suzuki Y., Miwa S., Shirai M., Ohba H., Murakami M., Fujita K., Suda T., Nakamura H., Hayakawa H., Chida K. Drug lymphocyte stimulation test in the diagnosis of adverse reactions to antituberculosis drugs // Chest. – 2008. – Vol. 134. – P.1027-1032. doi: 10.1378/chest.07-3088
11. Umeki S. Adverse effects of antitubercular drugs and significance of measurement of the drug-stimulating lymphocyte transformation rat // Jpn. J. Med. – 1989. – Vol. 28. – № 3. – P. 335-340. doi: 10.2169/internalmedicine1962.28.335
Review
For citations:
Averbakh M.M., Kosmiadi G.A., Gergert V.Ya., Panova L.V. A modified method of immunological diagnosis of intolerance to anti-tuberculosis drugs using additional stimulation of cultured lymphocytes with recombinant interleukin-2. Tuberculosis and socially significant diseases. 2024;12(1):25-29. (In Russ.) https://doi.org/10.54921/2413-0346-2024-12-1-25-29
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