The experience of serum lipoarabinomannan determination for the diagnosis of tuberculosis at the late stage of HI V infection
Abstract
The diagnostic significance of the serum lipoarabinomannan (LAM) content was studied in 106 patients at a late stage of HIV infection. Based on clinical and laboratory studies, patients were divided into a group of HIV-infected non-TB (n = 43) and HIV/TB (n = 63) groups, LAM was determined by ELISA using the LAM ELISA kit (SunLong Biotech Co., Ltd.). The level of LAM in patients with coinfection HIV/TB was significantly higher than in the HIV non-TB group (13.75 ± 1.5 pg / ml, CI ± 2.97 and 7.49 ± 0.5 pg / ml, CI ± 0.96, respectively; p = 0.000163). The sensitivity of the serum LAM-test in patients with HIV infection was 85.7%, and the specificity 79.1%. The level of LAM in the blood serum of HIV/TB patients is affected by the severity of immunosuppression, assessed by CD4+ blood lymphocyte count. In patients with a CD4+ lymphocyte count less than 100 cells/μL the serum LAM level was 14.8 ± 2.3 pg/ml, and at > 300 cells/μL 9.03 ± 0.8 pg/ml (p = 0.024). The determination of LAM serum should be considered as an additional criterion in the diagnosis of tuberculosis in the late stage of HIV infection.
About the Authors
N. L. Karpina
ФГБНУ «Центральный научно-исследовательский институт туберкулеза»
Russian Federation
Russian Federation
A. V. Dudchenko
ФГБНУ «Центральный научно-исследовательский институт туберкулеза»; ГБУЗ «Туберкулезная больница им. А.Е. Рабухина Департамента здравоохранения города Москвы»
Russian Federation
Russian Federation
M. M. Averbakh
ФГБНУ «Центральный научно-исследовательский институт туберкулеза»
Russian Federation
Russian Federation
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Review
For citations:
Karpina N.L., Dudchenko A.V., Averbakh M.M. The experience of serum lipoarabinomannan determination for the diagnosis of tuberculosis at the late stage of HI V infection. Tuberculosis and socially significant diseases. 2018;(2):49-54. (In Russ.)
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