Analysis of multidrug resistance protein gene expression in tuberculomas with different inflammatory activity
https://doi.org/10.54921/2413-0346-2024-12-1-19-24
Abstract
The aim of the work is to investigate the expression level of the main genes of multidrug resistance proteins of macroorganism somatic cells (MDR-SCM) – ABCB1, ABCC1, ABCG2 – in pulmonary tuberculomas with different inflammatory activity.
Materials and methods. Surgical material of pulmonary tuberculoma was obtained from 30 patients (16 men and 14 women). According to the results of histological examination and morphological analysis tuberculoma samples were divided into two groups: with moderate or severe inflammatory process. The level of expression of the ABCB1, ABCC1, and ABCG2 genes was evaluated for all samples by real-time polymerase chain reaction.
Results. The genes ABCB1, ABCC1, ABCG2 are expressed in the perifocal zone of tuberculomas and their expression level depends on the activity of inflammation. The highest expression level was found for the ABCB1 gene encoding the P-gp transporter protein.
About the Authors
M. V. Erokhina
Central Tuberculosis Research Institute;
Lomonosov Moscow State University
Russian Federation
Russian Federation
Moscow
L. N. Lepekha
Central Tuberculosis Research Institute
Russian Federation
Russian Federation
Moscow
E. Yu. Rybalkina
Central Tuberculosis Research Institute
Russian Federation
Russian Federation
Moscow
R. V. Tarasov
Central Tuberculosis Research Institute
Russian Federation
Russian Federation
Moscow
E. K. Tarasova
Central Tuberculosis Research Institute
Russian Federation
Russian Federation
Moscow
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Review
For citations:
Erokhina M.V., Lepekha L.N., Rybalkina E.Yu., Tarasov R.V., Tarasova E.K. Analysis of multidrug resistance protein gene expression in tuberculomas with different inflammatory activity. Tuberculosis and socially significant diseases. 2024;12(1):19-24. (In Russ.) https://doi.org/10.54921/2413-0346-2024-12-1-19-24
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