PHYLOGENETIC ANALYSIS OF MYCOBACTERIUM TUBERCULOSIS CLINICAL ISOLATES USING 35 VARIABILITY TANDEM REPEATS AND SIX SINGLE NUCLEOTIDE POLYMORPHISMS
Abstract
Clinical isolates of M. tuberculosis (n 330) were studied using MIRUVNTR methods, based on the analysis of 35 loci and analysis of point mutations. It is shown that each branch of the dendrogram of the molecular genetic relatedness of M. tuberculosis clinical isolates
contains isolates relating to the only one cluster group, defined by the point mutations analysis. All isolates of M. tuberculosis, relating to Beijing spoligofamily are in the same branch of the dendrogram. Isolates from the other spoligofamilies are missing in the group.
Isolates, belonging to LAM spoligofamily, are in the same branch of the dendrogram, which also includes isolates, belonging to T and S spoligofamilies. Isolates from T and Haarlem spoligo families comprise into several branches of the dendrogram, and T spoligofamily isolates belong to three SNP groups, while Haarlem isolates belong to the only one group, identified on the basis of point mutations analysis.
About the Authors
A. G. Bogun
ФБУН «Государственный научный центр прикладной микробиологии и биотехнологии» Роспотребнадзора
Russian Federation
Russian Federation
S. A. Blagodatskikh
ФБУН «Государственный научный центр прикладной микробиологии и биотехнологии» Роспотребнадзора
Russian Federation
Russian Federation
T. N. Mukhina
ФБУН «Государственный научный центр прикладной микробиологии и биотехнологии» Роспотребнадзора
Russian Federation
Russian Federation
N. V. Maiskaya
ФБУН «Государственный научный центр прикладной микробиологии и биотехнологии» Роспотребнадзора
Russian Federation
Russian Federation
I. G. Shemyakin
ФБУН «Государственный научный центр прикладной микробиологии и биотехнологии» Роспотребнадзора, г. Оболенск
Russian Federation
Russian Federation
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Review
For citations:
Bogun A.G., Blagodatskikh S.A., Mukhina T.N., Maiskaya N.V., Shemyakin I.G. PHYLOGENETIC ANALYSIS OF MYCOBACTERIUM TUBERCULOSIS CLINICAL ISOLATES USING 35 VARIABILITY TANDEM REPEATS AND SIX SINGLE NUCLEOTIDE POLYMORPHISMS. Tuberculosis and socially significant diseases. 2013;(2):5-11. (In Russ.)
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