Genotyping of isolates Mycobacterium tuberculosis from Ural
Abstract
According to suggested scheme genotyping of M. tuberculosis in Ural region of Russia is based on 2-step assay: the real-time PCR for differentiation of Beijing/non-Beijing isolates and further discrimination by MIRU-VNTR-typing using a set of 9 loci (MIRU26, QUB26, Mtub21, Qub11b, MIRU31, MIRU40 VNTR4120, VNTR3820, VNTR3232) for Beijing isolates, and 15 loci (Mtub04, ETRC, MIRU04, MIRU40, MIRU10, MIRU16, Mtub21, QUB11b, ETRA, Mtub30, MIRU26, MIRU31, Mtub39, QUB26, QUB4156) and/or spoligotyping for non-Beijing isolates.
About the Authors
T. V. Umpeleva
ФГБУ «Уральский НИИ фтизиопульмонологии» Минздрава России, г. Екатеринбург
Russian Federation
Russian Federation
A. A. Vyazovaya
ФБУН «НИИ эпидемиологии и микробиологии имени Пастера» Роспотребнадзора, г. Санкт-Петербург
Russian Federation
Russian Federation
M. A. Kravchenko
ФГБУ «Уральский НИИ фтизиопульмонологии» Минздрава России, г. Екатеринбург
Russian Federation
Russian Federation
N. I. Eremeeva
ФГБУ «Уральский НИИ фтизиопульмонологии» Минздрава России, г. Екатеринбург
Russian Federation
Russian Federation
O. V. Narvskaya
ФБУН «НИИ эпидемиологии и микробиологии имени Пастера» Роспотребнадзора, г. Санкт-Петербург
Russian Federation
Russian Federation
References
1. Cowan L.S., Diem L., Monson T. et al. Evaluation of a two-step approach for large-scale, prospective genotyping of Mycobacterium tuberculosis isolates in the United States // J. Clin. Microbiol.– 2005. – Vol. 43. – P. 688–695.
2. Iwamoto T., Yoshida S., Suzuki K. et al. Hypervariable loci that enhance the discriminatory ability of newly proposed 15-loci and 24-loci variable-number tandem repeat typing method on Mycobacterium tuberculosis strains predominated by the Beijing family // FEMS Microbiol. Lett. – 2007. – Vol. 272. – P. 282–283.
3. Kamerbeek J., Schouls L., Kolk A. et al. Simultaneous detection and strain differentiation of Mycobacterium tuberculosis for diagnosis and epidemiology // J. Clin. Microbiol. – 1997. – Vol. 35. – P. 907–914.
4. Kremer K., van Soolingen D., Frothingham R. et al. Comparison of methods based on different molecular epidemiological markers for typing of Mycobacterium tuberculosis complex strains: interlaboratory study of discriminatory power and reproducibility // J. Clin. Microbiol. – 1999. – Vol. 37. – P. 2607–2618.
5. Mokrousov I., Narvskaya O., Vyazovaya A., Millet J., Otten T., Vishnevsky B., Rastogi N. Mycobacterium tuberculosis Beijing genotype in Russia: in search of informative VNTR loci // J. Clin. Microbiol. – 2008. – Vol. 46. – P. 3576–3584.
6. Supply P., Allix C., Lesjean S. et al. Proposal for standardization of optimized Mycobacterial interspersed repetitive unit-variable-number tandem repeat typing of Mycobacterium tuberculosis // J. Clin. Microbiol. – 2006. – Vol. 44. – P. 4498-4510.
7. Van Embden J., Cave M., Crawford J. et al. Strain identification on Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology // J. Clin. Microbiol. – 1993. – Vol. 31. – P. 406-409.
Review
For citations:
Umpeleva T.V., Vyazovaya A.A., Kravchenko M.A., Eremeeva N.I., Narvskaya O.V. Genotyping of isolates Mycobacterium tuberculosis from Ural. Tuberculosis and socially significant diseases. 2013;(2):12-15. (In Russ.)
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