THE IMPACT OF MUTATIONS CONNECTED WITH RIFAMPICIN RESISTANCE ON THE M. TUBERCULOSIS STRAINS «FITNESS»
Abstract
To estimate the potential impact of mutations leading to the RIFresistance on the «fitness» of M. tuberculosis (MTB) we analyzed using TB-BIOCHIP («BIOCHIP IMB» Russia) the spectrum of single nucleotide polymorphisms (SNP) in rpoB gene in 309 MDR-MTB strains obtained from new-detected and 324 MDR-MTB strains obtained from previously treated TB-patients. It was found that in the group of MBT strains isolated from previously treated TB-patients was detected more mutant variants in comparison with MTB strains obtained from newdetected patients. Also MBT strains isolated from previously treated TB-patients had compensatory mechanisms aimed at severization of drug resistance due to additional mutations in the analyzed rpoB region. The strains with SNP 531 Ser → Leu prevailed in both groups (87,4% and 71,3%, respectively), that is clearly pointed to maintenance of «fitness» of strains with this SNP.
About the Authors
S. N. Andreevskaya
ФГБНУ «Центральный научно-исследовательский институт туберкулеза»
Russian Federation
Russian Federation
I. Yu. Andrievskaya
ФГБНУ «Центральный научно-исследовательский институт туберкулеза»
Russian Federation
Russian Federation
E. А. Kiseleva
ФГБНУ «Центральный научно-исследовательский институт туберкулеза»
Russian Federation
Russian Federation
E. E. Larionova
ФГБНУ «Центральный научно-исследовательский институт туберкулеза»
Russian Federation
Russian Federation
T. G. Smirnova
ФГБНУ «Центральный научно-исследовательский институт туберкулеза»
Russian Federation
Russian Federation
L. N. Chernousova
ФГБНУ «Центральный научно-исследовательский институт туберкулеза»
Russian Federation
Russian Federation
A. E. Ergeshov
ФГБНУ «Центральный научно-исследовательский институт туберкулеза»
Russian Federation
Russian Federation
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Review
For citations:
Andreevskaya S.N., Andrievskaya I.Yu., Kiseleva E.А., Larionova E.E., Smirnova T.G., Chernousova L.N., Ergeshov A.E. THE IMPACT OF MUTATIONS CONNECTED WITH RIFAMPICIN RESISTANCE ON THE M. TUBERCULOSIS STRAINS «FITNESS». Tuberculosis and socially significant diseases. 2016;(2):33-37. (In Russ.)
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