FORMATION OF RESISTANCE TO RIFAMPICIN IN MONOCYTE-MACROPHAGIC AND EPITHELIAL CELL LINES
Abstract
Our data demonstrate that drug resistance to subtoxic concentrations of rifampicin can form in monocyte-macrophagic and epithelial cell lines. The index of cytotoxicity IC50 (50% cell survival) increased 1.5–2.5 times during the first 3 months of drug administration. Rifampicin resistant cells were characterized by high functional activity of Pgp, one of the major MDR proteins mediating resistance to multiple structurally diverse drugs. In drug resistant macrophages, phagocytic activity increased. Thus, the index of phagocytosis of M. bovis-GFP increased 1.5 times after 3 month of drug administration. The phenomenon of resistance of monocytic and epithelial cell lines to rifampicin depended on the time of exposure to the drug and was reversible.
About the Authors
M. V. Erokhina
ФГБНУ «Центральный научно-исследовательский институт туберкулеза»
Russian Federation
Russian Federation
L. N. Lepekha
ФГБНУ «Центральный научно-исследовательский институт туберкулеза»
Russian Federation
Russian Federation
A. E. Ergeshov
ФГБНУ «Центральный научно-исследовательский институт туберкулеза»
Russian Federation
Russian Federation
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Review
For citations:
Erokhina M.V., Lepekha L.N., Ergeshov A.E. FORMATION OF RESISTANCE TO RIFAMPICIN IN MONOCYTE-MACROPHAGIC AND EPITHELIAL CELL LINES. Tuberculosis and socially significant diseases. 2016;(2):59-65. (In Russ.)
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